sars-cov-2 cpass surrogate virus neutralization test (svnt) kit  (GenScript corporation)

Journal: Vaccines

Article Title: The Humoral Immune Response Against COVID-19 Through Vaccination in Hemodialysis Patients

doi: 10.3390/vaccines13020170

Figure Lengend Snippet: Antibody titers from the first and second blood samples by the participants’ demographics.

Article Snippet: NAb was measured using the GenScript SARS-CoV-2 cPASS surrogate virus neutralization test (sVNT) kit (Genscript Biotech Corporation, Piscataway, NJ, USA) and spike-specific immunoglobulin G (IgG) was measured with the Euroimmun anti-SARS-CoV-2 IgG enzyme-linked immunosorbent assay (ELISA) (Euroimmun, Lübeck, Germany).

Techniques: Infection, Sampling

Journal: Vaccines

Article Title: The Humoral Immune Response Against COVID-19 Through Vaccination in Hemodialysis Patients

doi: 10.3390/vaccines13020170

Figure Lengend Snippet: Locally estimated scatterplot smoothing curve based on data from the first blood sample collection, illustrating the relationship of antibody levels as follows: ( A – C ) inhibition rates (%) for the surrogate virus neutralization test (sVNT) against the wild-type (WT) strain, ( D – F ) inhibition rates (%) against the Omicron variant, and ( G – I ) IgG levels. The curves are plotted against the number of days since COVID-19 infection ( A , D , G ), the last vaccination ( B , E , H ), and the last exposure to COVID-19 ( C , F , I ). The blue line represents the regression line, and the shaded gray area indicates the 95% confidence interval.

Article Snippet: NAb was measured using the GenScript SARS-CoV-2 cPASS surrogate virus neutralization test (sVNT) kit (Genscript Biotech Corporation, Piscataway, NJ, USA) and spike-specific immunoglobulin G (IgG) was measured with the Euroimmun anti-SARS-CoV-2 IgG enzyme-linked immunosorbent assay (ELISA) (Euroimmun, Lübeck, Germany).

Techniques: Inhibition, Virus, Neutralization, Variant Assay, Infection

Journal: Vaccines

Article Title: The Humoral Immune Response Against COVID-19 Through Vaccination in Hemodialysis Patients

doi: 10.3390/vaccines13020170

Figure Lengend Snippet: Locally estimated scatterplot smoothing curve based on data from the second blood sample collection, illustrating the relationship of antibody levels as follows: ( A – C ) inhibition rates (%) for the surrogate virus neutralization test (sVNT) against the wild-type (WT) strain, ( D – F ) inhibition rates (%) against the Omicron variant, and ( G – I ) IgG levels. The curves are plotted against the number of days since COVID-19 infection ( A , D , G ), the last vaccination ( B , E , H ), and the last exposure to COVID-19 ( C , F , I ). The blue line represents the regression line, and the shaded gray area indicates the 95% confidence interval.

Article Snippet: NAb was measured using the GenScript SARS-CoV-2 cPASS surrogate virus neutralization test (sVNT) kit (Genscript Biotech Corporation, Piscataway, NJ, USA) and spike-specific immunoglobulin G (IgG) was measured with the Euroimmun anti-SARS-CoV-2 IgG enzyme-linked immunosorbent assay (ELISA) (Euroimmun, Lübeck, Germany).

Techniques: Inhibition, Virus, Neutralization, Variant Assay, Infection

Journal: Microbial Cell Factories

Article Title: Oral administration of a recombinant modified RBD antigen of SARS-CoV-2 as a possible immunostimulant for the care of COVID-19

doi: 10.1186/s12934-024-02320-5

Figure Lengend Snippet: Neutralization assay of the hyperimmune sera obtained 30 days post-immunization of BALB/c mice with RBD-P and RBD orally ( A ) and intramuscularly ( B ). The y-axis corresponds to the observed percentage of the inhibition of HRP-conjugated RBD interaction with hACE2. Dashed lines represent manufacturers’ cutoff values (30%). The neutralization assay was performed in duplicate for each mouse serum, and per group. C+ is the positive control. Each boxplot presents the mean as an X, the median as the middle line, the interquartile range as box limits, and the 2.5th and 97.5th percentiles as the whiskers. For all variables with the same letter, the difference between the means is not statistically significant. If two variables have different letters, they are significantly different (p < 0.05). When there are two letters in a data set, this is not significantly different from the data sets with those letters

Article Snippet: 100 μL of the sample were placed on a capture plate with hACE2 protein from the cPass SARS-CoV-2 Surrogate virus neutralization test kit (Catalog L00847-A, Genscript, Ryswick, The Netherlands) for 15 min at 37 °C.

Techniques: Neutralization, Inhibition, Positive Control